Combinatorial Crispr/Cas9 For Suppression Of Latent Hiv-1 Provirus In Vitro

Ravichantar, Nithya (2020) Combinatorial Crispr/Cas9 For Suppression Of Latent Hiv-1 Provirus In Vitro. PhD thesis, Universiti Sains Malaysia.

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Although HAART effectively suppresses HIV-1 replication, its compromised effectiveness against non-B subtypes, the challenge of eliminating latent proviruses, life-long treatment, and viral resistance complicates the cure for HIV-1. CRISPR/Cas9, the latest genome editing tool, can overcome the limitations seen with HAART. By targeting the promoter of HIV-1 (LTR), CRISPR/Cas9 can disrupt the latent reservoirs. Here, we designed an improved CRISPR/Cas9 system, combinatorial CRISPR/Cas9 to concurrently knockdown multiple HIV-1 genes: structural (Pol and Gag), regulatory (Rev and Tat) and accessory genes (Vif). We investigated the efficacy of this tool as a therapy against different viral subtypes in a subset population . Our preliminary data, showed a huge decrease in viral load and we verified the safety of CRISPR/Cas9 in human cell lines.

Item Type: Thesis (PhD)
Subjects: R Medicine > R Medicine (General) > R5-920 Medicine (General)
Divisions: Institut Perubatan & Pergigian Termaju (Advanced Medical & Dental Institute (AMDI)) > Thesis
Depositing User: Mr Hasmizar Mansor
Date Deposited: 31 Mar 2022 07:08
Last Modified: 31 Mar 2022 07:08

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