Mintow, Alvieno Situl
(2011)
In vitro study on anti-amoebic activity of tualang
honey against entamoeba histolytica trophozoite.
Other.
Pusat Pengajian Sains Perubatan, Universiti Sains Malaysia.
(Submitted)
Abstract
Anti-bacterial properties of Malaysian honeys have been widely studied.
However, the anti-amoebic activity of local honeys against Entamoeba histolytica is still
not known. This study was conducted to preliminarily evaluate in vitro activity of one
local Malaysian honey which is tualang honey on the growth of E. histolytica
trophozoites. Anti-amoebic activity of tualang honey was assessed using a broth
dilution method and metronidazole as the control. Different concentrations of honey
[6.25% - 25% (weight/volume)] and metronidazole were tested against HM-1:1MSS
strain E. histolytica trophozoites in the 96-well flat-bottom microtiter plate under
anaerobic condition. The growth of trophozoites in each well was evaluated
microscopically using inverted microscope. Trophozoites were monitored for motility
and rounding-up and the numbers were scored accordingly. MIC was defined as the
lowest concentration of honey dilution at which a 1 + score (>90% rounded up, than the
control well) was obtained in the majority of the triplicate wells. It was found that
concentrations of tualang honey with a 1 + score ranged from 12.5% to 25% (w/v) while
concentrations with 2+ to 4+ scores were between 6.25% and 11.25% (w/v). Therefore,
the MIC of tualang honey was 12.5% (w/v). This study showed that the local Malaysian
tualang honey has an inhibitory effect on E. histolytica trophozoites in vitro. Thus, it can
provide a scientific basis for further evaluations on potential use of local honeys against
E. histolytica infection as well as studies related to determination of their anti-amoebic
properties.
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