Al-Amin, Md
(2021)
Isolation Of Antimigratory Chemical
Constituents From Curcuma Caesia, Curcuma
Aeruginosa Rhizomes And Dioscorea Bulbifera
Tubers Using Human Breast Cancer
Cell Lines.
Masters thesis, Perpustakaan Hamzah Sendut.
Abstract
Curcuma caesia, Curcuma aeruginosa and Dioscorea bulbifera are used
traditionally for the treatment of human ailments including cancer. The present study
was carried out to search for antimigratory compounds from C. caesia, C. aeruginosa
rhizomes and D. bulbifera tubers using breast cancer cell lines. The chemical
constituents from C. caesia and C. aeruginosa rhizomes have been isolated through
fractionation. The extracts of C. caesia and C. aeruginosa rhizomes showed
concentration-dependent inhibition of MCF-7 and MDA-MB-231 cells. Further study
showed that hexane and chloroform fractions of C. caesia rhizomes and chloroform
fraction of C. aeruginosa rhizomes are the bioactive fractions that significantly inhibit
the viability of breast cancer cells. The chromatographic separation afforded
germacrone (1), zerumbone (2), furanodienone (3), curzerenone (4), curcumenol (5),
zederone (6), curcumenone (7) and dehydrocurdione (8) from hexane fraction and
curcuminol G (9), curcuzederone (10), (1S, 10S), (4S,5S)-germacrone-1(10), 4-
diepoxide (11), wenyujinin B (12), alismoxide (13), aerugidiol (14), zedoarolide B
(15), zedoalactone B (16) zedoarondiol (17) and isozedoarondiol (18) from
chloroform fraction of C. caesia rhizomes. The chromatographic separation afforded
germacrone (1), furanodienone (3), curzerenone (4), curcumenol (5) and zederone (6)
from chloroform fraction of C. aeruginosa. Cell viability assay of these isolated
compounds further revealed that compounds 1-4 and 10, and compounds 1, 3 and 4
are the bioactive compounds of C. caesia and C. aeruginosa, respectively.
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