Mohd Yusri, Ahmad Fozi
(2009)
A Comparative In-vitro Study of the Viability of Cultured
Primary Normal Human Epidermal Keratinocytes on
Cellular and Acellular Human Amniotic Membrane.
Other.
Universiti Sains Malaysia.
(Submitted)
Abstract
Skin is the largest organ in human body. It plays important role to regulated and
maintain human body homeostasis. Based on this condition, burned skin needs
rehabilitation in order to reduce and avoid disturbances of body homeostasis. This study
aimed to assess the ability of Human Amniotic Membrane (HAM) as scaffold for the
growth of keratinocytes in-vitro. The HAM's epithelium had been removed using
enzymatic technique namely dispase and trypsin-EDTA. De-epithialized HAM (also
known as an acellular of HAM) was used as the main scaffold for the cultured primary
normal human epidermal keratinocytes (pNHEK). Cell viability was compared between deepithialized
HAM and HAM without de-epithialization (also known as cellular HAM) for
the three consequence days. Epithelium of HAM was successfully removed via dispase and
trypsin-EDT A, as confirmed by histochemical and cytochemical analysis. De-epitheliazed
HAM sustained better cell viability only at the first 24 hours compared with the HAM
without de-epithialization. However, viable cells were deserved only at 48 hours postseeding
of pNHEK on the HAM without de-epithialization. In conclusion, HAM can be deepithialized
using enzymatic technique. De-epithialized HAM and HAM without deepithialization
can sustain the growth of pNHEK. Result of this study will use to produces
specific kind of HAM for healing of skin burn.
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