Khor Kok, Pin
Distribution of allele frequencies of short
tandem repeats (STRs) DI6S539, D7S820 and D13S317 in random temiar population group of Malaysia.
Other.
Universiti Sains Malaysia.
(Submitted)
Abstract
Polymerase Chain Reaction based Short Tandem Repeat (PCR-STR) method provides an
unprecedented DNA profiling technique for forensic community in identification and
individualization purposes. In the analysis of biological material using DNA profiling
technique, the information regarding the distribution of allele frequency in a defmed
population is mandatory. This particularly necessary in paternity testing that involves
compilation of Paternity Index (PI), in which the allele frequency is required to indicate
whether the alleged father is the true biological father of the child. Validated 8TR loci have
been analysed extensively in various populations for forensic application. In this research
study, population genetic studies were carried out for three 8TR loci, namely D168539,
D7S820 and D13S317 for Temiar population by using a multiplex PCR reaction followed
by 4% polyacrylamide gel electrophoresis (PAGE) and silver staining. The heterozygosity
(H) for these three loci, i.e. D16S539, D78820 and D138317 are 0.9568, 0.9132 and 0.7427
respectively. Power of Discrimination (PD) for these three loci are 0.9135 (D16S539),
0.9331 (D78820) and 0.9050 (D13S317); PowerofExclusion (PE) was found to be 0.5192
(0168539), 0.6034 (078820) and 0.4053 (D13S317) respectively. The Cumulative
Discrimination Power (CDP) for the three 8TRs for Temiar population is 0.99903.
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