Khairun Nisa, Ibrahim Asri
(2009)
Screening of antiproliferative activity of
artocarpus sp. towards hela cell line and
caov-3 cell line.
Other.
Universiti Sains Malaysia.
(Submitted)
Abstract
Artocarpus sp. has been used as traditional folk medicine in Southeast Asia for treating
ulcer, abscess, and diarrhea. This species also been reported to be used for treatment of
inflammation, and malarial fever. In this study, three Artocarpus sp. extract were used to
treat two types of cancer cell lines that are cervical cancer cell line (HeLa) and ovarian
cancer cell line (CaOv-3) to screen the antiproliferative activity. The lowest (the best IC50
value) for inhibitory concentration at 50% of cells population (IC50) shows 6.3lJ.1g/mL.
Treatment of CaOv-3 with Artocarpus extract with the lowest ICso value at specific
incubation time that is 24 hr, 48hr, and 72hr resulted in inducing apoptosis have been
determined by Hoechst stain. Result showed the DNA fragmentation event in the treated
cells compared to untreated cells. Treatment of normal cell line, MDCK with the
Artocarpus extract with the lowest IC50 value showed no cytoselective effect in the extract.
However the extract inhibits cell growth at high concentration equal to I OJ.lg/mL. These
results proposed that Artocarpus sp.exhibit antiproliferative activity on HeLa and CaOv-3
which inducing apoptosis in CaOv-3 cells.
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