Khairul Nissa, Saidun
(2009)
Efficacy of indirect elisa using
crude soluble entamoeba histolytica
antigen for the diagnosis of
amoebic liver abscess.
Other.
Universiti Sains Malaysia.
(Submitted)
Abstract
Amoebiasis is an infection of a parasitic protozoan, Entamoeba histolytica. There are two
types of amoebiasis, intestinal amoebiasis and extraintestinal amoebiasis. Ten percent of
world population was infected by amoebiasis, and high mortality rate was recorded due to
amoebic liver abscess (ALA) second after malaria. Although it is treatable, it is usually too
late when detected. Many clinical tests and researches are underway to improve the
diagnosis of amoebic liver abscess. In this study, the efficiency of Indirect ELISA using
crude soluble antigen of E. histolytica for the diagnosis of ALA was investigated. The
serum samples used in this study were tested with Indirect Haemaaglutination Assay (IHA)
for ALA antibodies. The crude soluble protein was produced from whole cell lysate by
sonication. The concentration of the protein measured by Bradford assay was 5839.I
f..Lg/ml. The assay was optimized prior testing the efficacy. The optimum concentration of
the antigen was 20 f..Lg/mL, I :25 and I :500 were the optimum dilutions of primary and
secondary antibodies, respectively. Cut-Off Value (COV) that has been determined from
30 IHA negative ALA serum samples readings using Indirect ELISA was 0.5677. Optical
density (OD) reading of the serum sample tested with Indirect ELISA that less than this
point was regarded as negative. The sensitivity and specificity of the assay using crude
soluble antigen were 8I.3% and 96.7%., respectively.
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