Gan Chin, Chear
(2009)
The separation of phytochemical compounds of spica prunellae
and pericarpium of citrus reticula tae by using a reverse phase hplc method.
Other.
Universiti Sains Malaysia.
(Submitted)
Abstract
A reversed-phase HPLC-UV method was developed for the separation of
phytochemicals in water, n-hexane, and methanol extracts of two of the very common
used Chinese herbal medicine, Spica prunellae and pericarpium of Citrus reticulatae.
The phytochemical components of these extracts were separated by RP-HPLC on a
C-18 column and adjusting mobile phase (acetonitrile: methanol; 40: 60 v/ v), flow rate
at 0.8 mL/min.
The identification of phytochemicals separated by RP-HPLC with UV-detection at
280 nm and 320 nm is based on the comparison of peak retention times of some
previously published works. However, this needs to be confirmed by the use of standard
samples under the same experimental condition.
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