Chang Kah, Haw
(2009)
The Study of Morphologieal Features of Spermatozoa
under Ditl'erent Temperature by Seanning Electron Microscope.
Other.
Universiti Sains Malaysia.
(Submitted)
Abstract
Semen is the body fluid frequently found in sexual crimes. It is principally comprised of
spermatozoa suspended in seminal plasma Spermatozoa with distinct and characteristic
appearance can be detected and confinned microscopically. In this study, the
morphological features of spermatozoa under different temperature exposures were
observed and examined by scanning electron microscope which was greatly used for the
observation of discrete cell surfaces in three dimensional views at high magnification.
10 samples collected at Cytology section of Pathology Laboratory, Hospital Universiti
Sains Malaysia, were divided into three different portions and kept into three different
temperatures, including 55°C, 4°C and room temperature for a 5 day period. All the
samples were observed by scanning electron microscope after exposure to respective
temperature. Different scores were given to varied morphological features of spermatozoa
observed. Through the scoring of morphological features, statistical analysis was
conducted for the determination of any significant change. Result of the examination
showed significant difference in the morphological features of spermatozoa for both the
experimental samples as compared to the control samples.
After the high temperature exposure on the samples, significant changes in the shape and
surface of the bead, acrosome morphology and mid-piece of spermatozoa were found
Tapered end head was seen on heat exposure. In addition, depressions and fossa were
found on the surface of the head with damaged acrosome morphology. Bent mid-piece of
spennatozoa was also significantly observed with high temperature exposure.
On the other hand, significant changes were found with the samples on cooling. Tail and
mid-piece of spermatozoa differed significantly with control samples on exposure to low
temperature. Hair-pinned and severe coiled tails of spermatozoa were found in cooled
samples. Mid-piece of spermatozoa was also disrupted with thinning and bending on low
temperature exposure. Differences in the morphological features of spermatozoa on
exposure to different temperature can be used as a tool to establish the condition of a
sexual crime scene.
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