Othman, Nor Hayati
(2009)
Comparative study between flow cytometric and conventional HPE
method in multinodular goiter- USM experience.
Comparative study between flow cytometric and conventional HPE method in Multinodular Goiter- USM experience.
(Submitted)
Abstract
In HUSM, the most common thyroid diseases is multinodular goiter (MNG). In an earlier study of
300 consecutive cases it was noted that malignant transformation of MNG in our population was
high, 36.8%; higher that what is cited in the literature. We postulated that MNG is a precursor lesion
to the development of malignant thyroid diseases. The current study is an extension of the previous
work - is to determine if flow cytometry could be used to diagnose early malignant transformation
among patients who came in with MNG using the theoretical premise that flow cytometry detects
DNA changes in abnormal cells.
Progress in defining ·the role of flow cytometry in the assessment of solid tumors has been slow,
partly because of the greater histological complexity of solid tissues, and partly because of practical
difficulties in obtaining suitable material compared to hematological diseases. Over the past decade,
great advances have been made and flow cytometry is now used more frequently for measurement of
DNA content in solid tumors. Recently, the technique has been extended by the development of fl.ow
cytometric assays for other cellular components, such as membrane markers, oncoprotein and growth
factor receptors. There can be little doubt that it will become a valuable technique in the study of the
biology and treatment of cancer. Quantitative multiparameter flow cytometric analysis of surface
antigen and DNA has become a well established and reliable technique in the investigation of
lymphoreticular cells.
In solid tumors however, flow cytometry has mainly been used for DNA analysis, and more recently
for quantitative assay of nuclear antigens. Locally, no work has been done utilizing flow cytometric
determination of multinodular goiter using single cell suspension. We embarked on diagnosing MNG
using fl.ow cytometry and detect malignant transformation in thyroid cell suspension based on DNA
abnormalities.
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