Noor Hasfalina, Razali
Voltammetry Detection of Cotinine in Urine Sample.
Other.
Pusat Pengajian Sains Perubatan Universiti Sains Malaysia.
(Submitted)
Abstract
Cotinine is a major metabolite of nicotine. Exposure to nicotine is
measured by analyzing the cotinine level in the blood, saliva, or urine. Currents
methods for determination of cotinine are Gas Chromatography - Mass
Spectroscopy (GC-MS) and High Performance Liquid Chromatography (HPLC).
In this study, the determination of cotinine in urine sample was performed by
Differential Pulse Adsorptive Stripping Analysis (DPAdSV) for the first time .. The
urine sample was filtered with filter paper and diluted in 25 ml deionized water. 1
ml sample from the solution was spiked in 9 ml supporting electrolyte, Britton
Robinson Buffer pH 7 and determined using optimum parameters such as initial
potential, E1 at -1.2, end potential, Et at -1.82, scan rate at 0.30 V/s, deposition
potential 0 V, equilibration time 30 seconds and deposition time 0 V. The
validation of methodology was described by obtaining regression equation for its
linearity by plotting the calibration curve of peak height, ip versus series
concentration of cotinine standard solution. A linear graph with regression
equation of Y = 1 0.922x + 2.066, correlation coefficient of 0.991, standard
deviation of +0.938, sensitivity of 10.922 uA/ppm and limit of detection of 0.258
ppm was obtained. 10 smoker urine samples were analyzed. Eight samples
including the non-smoker urine sample showed the presence of cotinine in
different concentration level with a range of 0.31 ppm to 29.15 ppm. Cotinine is
not detected in the two remaining urine samples.
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