Nazrul Anuar, Ali
(2008)
Screening of potential microorganisms for the
production of novel cyclodextrin
glycosyltransferase (CGTase)enzyme.
Other.
Pusat Pengajian Sains Perubatan Universiti Sains Malaysia.
(Submitted)
Abstract
Ten soil samples were collected from the vicinity of restaurant's drain where the wastewater
contain remaining starch food constituent was filtered and drained away, housing estate ditch and
trash waste disposal area around Kubang Kerian. They were screened for potential CGTase
enzyme producer by culturing in media containing cassava starch and nutrient agar. A total of 40
colonies have been successfully isolated in this study by detecting the production of clearance
zone around them, and those colonies were selected as CGTase producing bacteria. The CGTase
producing bacteria were identified as Bacillus species by microscopy observation. The bacteria
was grown in production medium containing 1.5% (w/v) cassava starch, 0.4% (w/v) (NH4)2S04,
O.lM phosphate buffer (pH 7.0), 0.002% (w/v) MgS04 and 0.002% (w/v) FeS04 for the
production of CGTase enzyme. The supernatant obtained after centrifugation at 3000 g for 15
minutes was used as crude enzyme. The activity of CGTase production was evaluated
qualitatively by observing the production of clearance zone around the colony and quantitatively
by determining the dextrinizing activity based on the method described by Fuwa, (1954) and
Matsuzawa et al., (1975) with slight modification.
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