Baharudin, Abdullah
(2008)
The effects of bovine bone scaffold on the microscopic
biological response of human chondrocytes.
Universiti Sains Malaysia.
(Submitted)
Abstract
Introduction
In the creation of cartilaginous tissue, the choice of suitable scaffold remains a
great challenge. The current available scaffold either natural or synthetic still
does not meet the requirement of a scaffold for cartilage tissue engineering. The
ideal scaffold provides mechanical stability to the individual cell as a construct or
transitional framework before synthesis of new extra cellular matrix.
Objective
The aim of this study was to produce bovine bone as a tissue engineering
construct for cartilage reconstruction and to determine the effects of bovine bone
on biological human chondrocyte in vitro.
Method
Human chondrocytes were cultured and seeded into bovine scaffold with seeding
density of 1 x 105 cells per 1 00 ul I scaffold and incubated for 24 hours, 2 days, 5
days and 7 days. Proliferation and viability of the cells were measured by
mitochondrial dehydrogenase activity (MTT assay), adhesion study was analyzed
using Scanning Electron Microscopy (SEM) and differentiation study was
analyzed by lmmunoflorescent staining using Confocal Laser Scanning Electron
Microscopy (CLSM). Result
The data showed the presence of proliferation and viability of the cells on the
scaffolds by MTT method within 24 hours to 7 days observed. SEM pictures
revealed presence of chondrocytes located on the scaffolds, showed increasing
number of cell within the days and that cells readily grew on the surface and into
the open pores of the scaffold. lmmunoflorescent staining detected collagen type
II on the scaffolds which was increasing within the days.
Conclusion
The results showed the potential of bovine bone as three dimensional scaffold for
cartilage tissue engineering because of the good cells proliferation, attachment,
maturity, non toxic, safe, easily resourced and relatively cheap.
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