Utar, Zulkhurnain
(2014)
The Effects Of Mitragynine From
Mitragyna Speciosa On The Mrna
Expression Of Cox-1 And Cox-2 In
Lipopolysaccharide-Stimulated
Raw264.7 Macrophage Cells.
Masters thesis, Universiti Sains Malaysia.
Abstract
Mitragyna speciosa Korth (Rubiaceae) is one of the medicinal plants used
traditionally to treat various types of diseases especially in Thailand and Malaysia.
Its anti-inflammatory and analgesic properties of its crude form are well documented.
In this study, the cellular mechanism involved in the anti-inflammatory effects of
mitragynine, the major bioactive constituent, was investigated. The effects of
mitragynine on the mRNA and protein expression of COX-1 and COX-2 and the
production of prostaglandin E2 (PGE2) were investigated in LPS-treated RAW264.7
macrophage cells. Quantitative RT-PCR was used to assess the mRNA expression of
COX-1 and COX-2. Protein expression of COX-1 and COX-2 were assessed using
Western blot analysis and the level of PGE2 production was quantified using
Parameter™ PGE2 Assay (R&D Systems).
Actions (login required)
 |
View Item |
