Detection of anti-giardia IgG in selected orang asli serum samples

Sanyang, Abdoulie M. (2019) Detection of anti-giardia IgG in selected orang asli serum samples. Masters thesis, Universiti Sains Malaysia.

PDF - Submitted Version
Download (2MB) | Preview


Giardiasis is a water-borne parasitic disease caused by the enteric protozoan, Giardia lamblia. The disease is prevalent in Orang Asli settlements due to the risk factors such as substandard sanitary system, poor hygiene practice and unclean water supply. Individuals harbouring the parasite mostly remain as asymptomatic carriers, but the disease may progress to mild, moderate or severe clinical intestinal symptoms. To better control the disease, epidemiological data reflecting on the range of affected population plays a pivotal role. The currently available epidemiological data of giardiasis in Orang Asli inferred only to active infection at a single timepoint via cross-sectional study design. The reported data might be underestimating the population at risk or exposed to giardiasis. Hence, the present study aimed to detect the presence of anti-G. lamblia IgG in selected Orang Asli serum samples via inhouse CSA-IgG-ELISA. A total of 150 archived serum samples were used in the present study. Crude soluble antigen was prepared from axenically grown G. lamblia and used for development of in-house CSA-IgG-ELISA. The in-house ELISA was set up with coating antigen of 10 μg/mL, serum dilution of 1:50 and HRP-conjugated secondary antibody of 1:6000. From the analysis, 68.7% showed ELISA optical density (OD) at 450nm readings of at least 1.000; it was at least three-fold of previously reported cases by PCR or microscopy. There were no associations between ELISA OD450nm readings and subjects’ demographic variables i.e. age and genders. Similarly, there were no significant difference found between ELISA OD450nm readings of RT-PCR positive and negative serum samples. The finding suggested seroprevalence of anti-giardia IgG could be a better indicator for estimating population exposed to giardiasis.

Item Type: Thesis (Masters)
Uncontrolled Keywords: Giardia
Subjects: R Medicine
Divisions: Kampus Kesihatan (Health Campus) > Pusat Pengajian Sains Perubatan (School of Medical Sciences) > Thesis
Depositing User: Mr Abdul Hadi Mohammad
Date Deposited: 05 Aug 2021 04:01
Last Modified: 05 Aug 2021 04:01

Actions (login required)

View Item View Item