Fong Slew, Wai
(2008)
A study of peroxisome proliferator-activated receptor (PPAR) alpha in patients with ischemic heart disease and heal thy volunteers.
Other.
Universiti Sains Malaysia.
(Submitted)
Abstract
Ischemic heart disease (IHD) is the most common cause of cardiovascular
mortality in Malaysia and around the globe. New novel biomarker, Peroxisome
Proliferator-Activated Receptors-Alpha (PPAR-a) was investigated in this study
to give significant contribution to early detection of IHD. In this study, we
investigated the mRNA expression and protein levels of both PPAR-a and
C-Reactive Protein (CRP) genes in the peripheral blood of IHD patients (n=10)
and apparently healthy volunteers (n=1 0). The mRNA levels of PPAR-a (native
and truncated) and CRP were determined by reverse transcription-polymerase
chain reaction. The protein expression was evaluated by Western blot and
measured by Enzyme linked immunosorbent assay (ELISA). The CRP mRNA
expression and serum levels increased significantly (p<0.05) in IHD patients
compared to normal volunteers. There was presence of both native active and
truncated inhibitory PPAR-a protein in human blood leukocytes. The mRNA
expressions of both native and truncated variants of PPAR-a were lower (p
<0.05) in IHD patients group than those in the normal volunteers group.
However, there was no significant difference (p>0.05) in PPAR-a protein level
between IHD patients and normal volunteers. These findings suggest that
mRNA expression level of both PPAR-a variants is decreased in IHD patients.
An over-expression of the truncated inhibitory isoform of PPAR-a protein may be
involved in the pathogenesis of IHD. Further study will be conducted to elucidate
this matter.
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