Masran, Siti Nor Ain Seri
(2019)
Phylogenetic And Genetic Analysis Of Tropical Bed Bug Cimex Hemipterus (F.) Population In Peninsular Malaysia.
PhD thesis, Universiti Sains Malaysia.
Abstract
The tropical bed bugs, Cimex hemipterus (Fabricus) wingless insects, is
recognized scientifically as a significant public health problem. The association of
bed bug populations between isolates in terms of geographical location with in-depth
and significant understanding is important for developing effective control strategies.
The study was conducted to understand the genetic reproduction pattern of the C.
hemipterus population in terms of allelic variation, genetic diversity, population
structure and phylogeography among tropical bed bug populations in Peninsular
Malaysia. The phylogenetic study was carried out by using mitochondria cytochrome
oxidase subunit I (COI) on twenty-two tropical bug populations with three pooled
bed bugs respectively. The analysis of COI gene data produced a phylogenetic tree
with a major monophyletic clade consisting of two groups of Ch01 and Ch02. The
Ch02 group was consist of Bandar Hilir populations, in contrast to other populations
that make up a single cluster. The Ch01 group shows similarities with some of the
COI genes of the C. hemipterus from Thailand, suggesting the similarity of
population variation. However, the variation of the observed COI gene is low. Next,
from NGS analysis, 50 microsatellite markers were developed, with seven
microsatellite markers selected for the polymorphisms study. A total of 220-bed bugs
samples were genotyped with seven species-specific polymorphic microsatellite
markers and showed 6-14 allele per locus, with high genetic diversity observed
among populations. The mean observed and expected heterozygosity of the overall
population were 0.280 and 0.828.
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