Construction of baculovirus transfer vectors for insect cell expression of human choline and ethanolamine kinase isoforms

Chang Chia, Than (2008) Construction of baculovirus transfer vectors for insect cell expression of human choline and ethanolamine kinase isoforms. Other. Universiti Sains Malaysia. (Submitted)

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Abstract

Phosphatidylcholine and phosphatidylethanolamine are the most abundant phospholipid in eukaryotic cell membrane. Hwnan choline kinase and ethanolamine kinase play a very important role in the biosynthesis of phosphatidylcholine and phosphatidylethanolamine by catalyzing in the first phosphorylation step of the pathway. In this study, genes encoding hwnan choline kinase-P (hCK-P) and ethanolamine kinase-2P (hEK-2P) isoform gene fragments were inserted into newly constructed plasmid. The plasmid was constructed from commercially purchased transfer plasmid pBAC4x-1. Open reading frames (ORF) ofhCK-P and hEK-2P were introduced into the downstream of polyhedrin promoter for expression as histidine-tagged (his-tag) proteins. The constructs were ready for homologous recombination with Autographa californica Nuclear Polyhedrosis Virus (AcNPV) genome. Insect cells infected with the recombinant AcNPV were expected to overexpress the hCK-P and hEK-2P proteins.

Item Type: Monograph (Other)
Uncontrolled Keywords: Eukaryotic cell
Subjects: R Medicine > R Medicine (General)
Divisions: Kampus Kesihatan (Health Campus) > Pusat Pengajian Sains Perubatan (School of Medical Sciences) > Monograph
Depositing User: Mr Husnan Budin
Date Deposited: 18 Apr 2021 06:13
Last Modified: 18 Apr 2021 06:13
URI: http://eprints.usm.my/id/eprint/48914

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