Muhammad Hafiznur, Yunus
(2008)
Cloning and characterization of bovine growth hormone (bGH) gene from Malaysian gaur (Bos gaurus hubbacla).
Other.
Universiti Sains Malaysia.
(Submitted)
Abstract
Total genomic DNA from Malaysian gaur (Bos gaurus hubbacla) was isolated from whole
blood. The genomic DNA was then subjected to PCR amplification using specific primers
for bovine growth hormone gene (bGH). This PCR product which represents part of the
bGH gene was cloned into pCR® 2.1 TOPO® cloning vector before sent for sequencing
analysis. The nucleotide sequence of the gene coding regions was found to be identical with
that of GenBank database sequence of Bos gaurus growth hormone mRNA sequence
except for two nucleotides position (position 101 and 128). The current database of Bos
gaurus growth hormone mRNA sequence at GenBank showed one unknown nucleotide at
position 128 which denoted as N in the sequence. The finding of this study determined that
the nucleotide base at position 128 is cytosine (C). The entire cloned gene sequence is 665
bp in length and contains 333 bp coding regions that code for 110 amino acid residue. The
in-silico restriction mapping site analysis carried out in this study revealed that 70 enzymes
produce unique site when they cut the sequence. A comparison of the cloned Malaysian
gaur bGH gene with Bos taurus (local cattle) growth hormone mRNA sequence also
showed a high homology between both sequences.
Actions (login required)
 |
View Item |
