Shobana Devi, Murugiah
(2008)
Production of highly immunogenic/ hydrophilic regions
of naiha gene using assembly pcr.
Other.
Universiti Sains Malaysia.
(Submitted)
Abstract
Influenza pandemics resemble major natural disasters. According to the list that has been
published by World Health Organization (WHO), the number of total cases of avian influenza is
387cases and 245 of those cases had cause deaths. The number of total cases and deaths shows
that there was an increased pandemic threat. Justifiably, this had caused the world-wide public
concern and it also explains the need for vaccine production for this Avian Influenza. The main
antigenic determinants of influenza A and B viruses are the haemagglutinin (HA) and
neuraminidase (NA) transmembrane glycoproteins. The activities ofHA and NA are functionally
linked and make them the most potential candidates for the avian influenza disease vaccine in
human. In this study, highly immunogenic/ hydrophilic regions ofNAIHA gene was constructed
using assembly PCR. The synthetic gene was constructed using assembly PCR and cloned into
pCR®2.1-TOPO®vector. The recombinant plasmid isolated from the colony was send for
sequencing and alignment result shows that there were three errors in the synthetic gene being
constructed. PCR-based site directed mutagenesis technique must be performed to repair those
errors. Although the synthetic gene was not developed as originally planned, this study has
generated data that would be useful towards achieving that goal in the future.
Actions (login required)
 |
View Item |
