Noraini, Mat Yunus
(2008)
Construction of dna vaccine expressing msp-1c of plasmodium falciparum.
Other.
Universiti Sains Malaysia.
(Submitted)
Abstract
Malaria is one of the most threatening parasitic diseases, being endemic in most of the
tropical and subtropical countries. This problem becomes severe with the rapid spread of
drug resistant Plasmodium falciparum, development of resistance to insecticides by
Anopheles mosquito, poor hygienic care, socioeconomic problems and transition of
people. Therefore an effective and universal malaria vaccine against Plasmodium
falciparum should be developed. A great deal effort of researchers nowadays has been
focused on vaccines targeting at the asexual blood stage due to prolong life cycle within
the blood and it can directly reduce morbidity and mortality associated with malarial
disease in human4 One of the vaccine candidates from asexual blood stage is a 19 kDa Cterminal
region of merozoite surface protein 1 (MSP-1C). MSP-1C protein has been
considered as a good candidate for malaria vaccine development due to their obvious
accessibility by host's immune response. The purpose of this project is to construct a DNA
vaccine expressing MSP-1 C of P. falciparum, In order to develop this vaccine; MSP-1 C
fragment was amplified from plasmid pNMN016 by using polymerase chain reaction
(PCR). The MSP-1C fragment was ligated into pVAX1, a plasmid vector that is designed
for use in the development of DNA vaccines. Hopefully, the development of DNA vaccine
against P. falciparum would decline the number of malaria cases worldwide.
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