Lee Hui, Hua
(2008)
Evaluation of the conventional test methods for diagnosis of methicillin-resistant staphylococcus aureus (MRSA).
Other.
Universiti Sains Malaysia.
(Submitted)
Abstract
Methicillin-resistant Staphylococcus aureus (MRSA) is a multiple drug-resistant
bacterium, which responsible for nosocomial and community-acquired infection. This
study compared the mannitol salt agar-oxacillin (MSO), mannitol salt agar-cefoxitin
(MSC), oxacillin resistance screening agar base (ORSAB), chromogenic agar medium
(CHROMagar), and MRSA-screen latex agglutination test to mecA PCR method as
the "gold standard" assay in order to determine the most simple, reliable and cost
effective test method for diagnostic purpose. 58 MRSA strains were isolated form 144
samples collected from HUSM. The MSO, MSC, ORSAB, CHROMagar, and
MRSA-screen gave sensitivity with 94%, 95%, 97%, 95% and 93% respectively,
specificity with 98%, 87%, 97%, 95% and 99% respectively, positive predictive value
(PPV) with 98%, 83%, 95%, 93% and 98% respectively, negative predictive value
(NPV) with 95%, 96%, 98% 97% and 96% respectively, and cost with RM1.24, RM
1.38, RM 1.42, RM 8.50 and RM 12.50 respectively for one sample. All the test
methods were agreed with PCR. In conclusion, ORSAB have the highest diagnostic
value, followed by MSO, MRSA-screen, CHROMagar and lastly MSC. The MSO
was the cheapest test method, followed by MSC, ORSAB, CHROMagar and then
MRSA-screen. ORSAB can be the most suitable diagnostic test method with high
diagnostic test value and low cost.
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