Yusop, Nur Hafiza Md
(2020)
Genotype and allele frequency of human neutrophil antigens in Acheh, Kedah, Mandailing, Minangkabu and Pattani Malays.
Masters thesis, Universiti Sains Malaysia.
Abstract
Human neutrophil antigens (HNAs) have been reported to involve in
pathogenesis of clinical conditions such as neonatal alloimmune neutropenia (NAN)
and transfusion-related acute lung injury (TRALI), thus knowledge on local
distribution of HNA alleles play significant roles in disease studies. The present
study involves polymerase chain reaction (PCR) using sequence specific primer
(SSP) typing of HNA-1, -3, -4 and -5 loci in 194 high molecular weight genomic
DNA samples extracted from blood samples of Acheh (n=35), Kedah (n=30),
Mandailing (n=47), Minangkabau (n=47) and Pattani (n=35) Malay individuals with
the target of obtaining data for population review along with HNA incompatibility
risk investigation. In general, datasets obtained in this study shows HNA-1a/1a, -
3a/3a, -4a/4a and -5a/5a were the most frequent genotypes observed in the Malay
sub-ethnic groups. However, HNA-1a/1b was recorded as the most frequent
genotype for HNA-1 system in Kedah, Mandailing and Pattani Malays while HNA-
5a/5b was logged as the most common HNA-5 genotype in Acheh and Pattani
Malays. The HNA population datasets collected in the present survey are similar to
those from the earlier study on Malay sub-ethnic groups (Kelantan, Banjar, Jawa,
Banjar and Bugis Malays), yet, significant differences were observed between Malay
sub-ethnic groups and HNA datasets reported for Orang Asli Semang (Bateq, Kensiu
and Lanoh) and Senoi (Che Wong and Semai). This observation reflects the uniquegene pools of different population groups in Peninsular Malaysia, which largely
associated with their different origins. Statistical analyses on the HNA datasets also
showed a low risk of HNA-related transfusion and gestation alloimmunizations for
HNA-1c, -3a, -4a and -4b and these risk assessments applied to any pairs of
mother/father and donor/patient population groups. However, probability of
alloimmunization for other HNAs (e.g. HNA-1a, -1b, -5a and -5b) is high even for
couples or donor and recipient of similar ethnic background. In conclusion, the
present study has successfully genotyped four HNA systems in five Malay sub-ethnic
groups and these HNA datasets provide valuable source of information for studying
population history and health in Peninsular Malaysia.
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