Nurhidayah, Ab. Rahim
(2008)
Establishment of glioma cell line from
freshly clinical sample of HUSM.
Other.
Universiti Sains Malaysia.
(Submitted)
Abstract
A human malignant continuous cell line, named as GBM N was established from a patient
diagnosed with recurrents glioblastoma multiforme which is grade IV glioma. This primary
cell line has been serially subcultured until 6th passage in the standard culture media that
presenting the existence of various marker. The aim of this study is to assess the
morphology of cultured glioblastoma multiforme cells as well as to apply the confirmatory
technique such as immunocytochemistry and western blotting method in order to confirm
the biological and immunological characteristic of gliomas. Immunocytochemistry finding
indicate this cell line retain the expression of Glial Fibrillary Acid Protein (GF AP) despite
some previous study show no GF AP are exist in established glioma cell line. However, our
study suggests, to prove the existence of GF AP in glioma cell line; we need to culture and
subculture until 50th passage in where only the specific protein marker will be presented.
Another technique used to confirm the establishment of glioma cell line is western blotting
method which serves as total protein study in explaining the biology of tumor as well as to
identify the protein that are very potential to cause human disease.
We believe that the information regarding the establishment of GBM IV cell line will give
benefits to all research community especially who have some interest to cellular and
molecular study of cancer with the main purpose to improve glioma's treatment.
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