Chia, Phee Phee
(2008)
A study of human choline kinase a (hCK-a)
in patients with ischemic heart disease
(ihd) and heal thy volunteers.
Other.
Universiti Sains Malaysia.
(Submitted)
Abstract
Ischemic heart disease (IHD) is predicted to become the dominant cause of
mortality worldwide by 2030. As a silent yet eventually fatal disease, prevention
and early detection through discovery of cardiac biomarker are the best resolution
for IHD. Human choline kinase a (hCK-a) was investigated for its possibility as a
cardiac biomarker in this study. The aim of this study is to investigate the
differences in the protein level and mRNA expression of hCK-a between IHD
patients and healthy controls. Blood samples from 10 IHD patients and 10 healthy
volunteers were analyzed for mRNA expression using RT-PCR. Western blot was
performed as a checkpoint for detection of hCK-a protein in blood. Subsequently,
ELISA test was conducted to measure the protein concentration of hCK-a protein.
C-reactive protein (CRP) was used as a standard reference in this study to provide
a parameter to distinguish between both study groups. Statistical analysis was
performed to investigate the relationship between hCK-a with IHD. Parametric test
was performed using independent t-test meanwhile non-parametric test was
performed using Mann-Whitney test. As anticipated, CRP was found to be
significant higher in expression both in mRNA level and protein level (Independent
t-test, p<0.001; Mann-Whitney test, p= 0.019). In this study, a significant lower
level of hCK-a2 mRNA expression in IHD patients was revealed (Independent ttest,
p=0.032). However, total hCK-a mRNA expression was found to have no
significant different between IHD patients and healthy volunteers (Mann-Whitney
test, p=0.705). Protein expression level of hCK-a was found to be significantly
lower in lHD patients as compared to healthy volunteers (Mann-Whitney test,
p=0.014). Albeit preliminary, this study found that there may be a profound
relationship between hCK-a and IHD.
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