Mohd Hamzah, Mohd Nasir
(2006)
Subcloning of Mycobacterium tuberculosis KatG gene in bacterial
expression system".
Other.
Universiti Sains Malaysia.
(Submitted)
Abstract
KatG is the gene in Mycobacterium tuberculosis which responsible for
encoding of catalase peroxidase enzyme to enable its survival in host macrophage.
KatG also reported to play an important role in enabling the killing effect of the antituberculous
pro-drug, isoniazid (IN H). The mutation of this gene has been reported
to create resistancy of Mycobacterium tuberculosis toward INH treatment. Since
the killing effect of the drug has a significant relation with this gene, the structure
and properties of KatG should be study for further information.
EcoRI restriction enzyme was found to produce 2252 bp of DNA product.
The gene was then sliced out from pCR®2.1-TOPO by using EcoRI restriction
enzyme and ligated into a high-level protein expression vector, pRSET for
expression assay. The recombinant sequenced has been confirmed by EcoRIPvull
restriction analysis. The gene has also been sequenced by cycle sequencing
and the primers are M13F, M13R, KG3 and KGS.
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