Goh, Yung, Shin
(2006)
Analysis of biologically active constituents from
water and methanol soluble extracts of reishi mushroom
(Ganoclerma lucidum)".
Other.
Universiti Sains Malaysia.
(Submitted)
Abstract
Ganoderma lucidum is one of the treasures of Chinese medicine. It has attained
an unparalleled reputation in the Orient as the ultimate herbal substance. In this
study, a rapid and efficient method for the extraction of bioactive molecules in
Ganoderma Jucidum is described. This method is based on the extraction of
Ganoderma lucidum with hot water, methanol and a 1:1 v/v mixture of hot
water-methanol. The FT-IR spectra of Ganoderma lucidum and its extracts were
recorded in potassium bromide (KBr) over the range of 4, 000-400 cm-1
. The UVVisible
spectrophotometric measurements were performed in water and
methanol over the range of 200-800 nm. Bioactive elements in the aciddigested
Ganoderrna lucidum samples were determined by flame atomic
absorption spectroscopy (AAS). The FT-IR spectra of Ganoderma lucidum and
its extracts exhibited characteristic absorption bands with significant difference
in their number and position. The infrared spectral bands indicated presence of
functional groups attributed to polysaccharides, amino acids, vitamins, terpanes
and enzymes moieties. The UV-Visible spectra, however, could not give much
information on the structure of the compounds extracted, due to very complex
nature of biological molecules. The concentration of elements determined in
Ganoderma lucidum by flame AAS was found for Na 12.5 ± 0.001 mg/L, K
210.4 ± 0.003 mg/L, Ca 23.9 ± 0.001 mg/L, Mg 7.1 ± 0.005 mg/L, Zn 1.9 ±
0.001 mg/L and Fe 1.2 ± 0.004 mg/L.
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