Rabail, Nasir Aziz
(2006)
The effects of Prostaglandin J2 metabolite on selected apoptotic signaling molecules in UeLa cells.
Other.
Universiti Sains Malaysia.
(Submitted)
Abstract
Cancer of the cervix is one of the most rapidly increasing malignancies throughout
the world and rated as the second most common cancer in Malaysian women. A
ligand-dependent nuclear receptor, peroxisome proliferator-activated receptor
gamma (PPARy) has been reported to be expressed in various cancer cells
including breast, prostate, co lorecta I and cervical cance:r. Recently, down
regulation of PPARy has been reported in human cervical carcinoma.
Investigations have demonstrated that activation of PPARy is linked to growth
inhibition of various cancers by induction of necrosis, apoptosis and growth arrest.
This study aims to identify the effect of selective PPARy ligand, 15-Deoxyprostaglandin
J2 (15d-PGJ2) on selected apoptotic molecules in the cervical cancer
cell line, HeLa. HeLa cells were cultured and treated with an ECso dose of 15dPGJ2.
These cells were harvested at every sixth hour of incubation period starting
from 0 - 72 hours. Western bJot analysis revealed that Aid expression (anti
apoptotic) was down regulated, while caspase 9 and caspase 3· (both are pro
apoptotic) expression was up regulated. This finding provides significant evidence
on the mechanism of apoptotic action of PPARy ligand that is potentially useful as
a chemotherapeutic agent.
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