Cytotoxicity testing of chitosan derivatives on primary cultured fibroblast

Nurul Firdaus, Abdul Hadi (2006) Cytotoxicity testing of chitosan derivatives on primary cultured fibroblast. Other. Universiti Sains Malaysia. (Submitted)

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Cbitosan has been used in several studies as wound healing accelerator. This study was designed to determine the cytotoxicity of cbitosan on primary cultured human fibroblasts. Human skins were obtained from surgical specimen of consented donor and primary cultured fibroblasts were established. The fibroblasts were incubated in the absence or presence of increasing concentrations of Oligo Chito 1% and Oligo Chito 5% for 24, 48 and 72 hours. Subsequently, MIT reagent was added and 4 hours later the purple crystals produced were dissolved in DMSO. Then, cells viability was read using ELISA plate reader. Cells viability was used as a marker for toxicity. The results showed that cell viability was dependent on concentration. The cell viability decreased when high concentrations of chitosan were used. Prolonged incubation period also caused decreased cell viability. Analysis of chitosan effects on cell culture is useful as a screening tool for their potential in vivo activity as wound healing agents.

Item Type: Monograph (Other)
Uncontrolled Keywords: Healing accelerator
Subjects: R Medicine
Divisions: Kampus Kesihatan (Health Campus) > Pusat Pengajian Sains Kesihatan (School of Health Sciences) > Monograph
Depositing User: Mr Husnan Budin
Date Deposited: 10 Mar 2021 08:37
Last Modified: 16 Mar 2021 04:59

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