Ismail, Siti Balkiah
(2006)
Classification of Haplogroup F in Chinese Population of
Peninsular Malaysia.
Other.
Universiti Sains Malaysia.
(Submitted)
Abstract
mtDNA is passed from one generation to the next, essentially unchanged,
solely through the maternal line of a family. A total of 44 samples were randomly
collected from USM Kampus Kesihatan, Kubang Kerian, Ketantan. mtDNA was
extracted from the buccal swabs. All samples were subjected to PCR by using a
set of primer, 12233-F and 12630-R to amplify a 397 bp fragment of mtDNA
coding region. This fragment was then digested using Hinc II enzymes for
determination of the Hinc II site loss or site gain at nucleotide position 12406 for
all samples. Samples which did not cut by the Hinc ll enzymes were classified
into the F haplogroup. Only 2 samples which are 4.5 % from 22 samples
analysed were found to belong to haplogroup F.
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