Seyram, Elom Achoribo
(2019)
Elucidation Of The Mechanism
Involved In The Proliferation And
Migration Activity Of Cyperus
Esculentus Lativum In Mda-Mb-231 And
Mcf7 Breast Cancer Cell Lines.
PhD thesis, Universiti Sains Malaysia.
Abstract
Cyperus esculentus L., is a tuber of family Cyperaceae which produces
rhizomes from the base. It is used mostly as flour and oil in the food industry. In Ghana,
it is mostly consumed raw as a snack because of its milky and sweet taste. The oil
contains beta-sitosterol, oleic acid and quercetin, which are the compounds found to
have anticancer property on various cancer cell lines. This research aimed to
investigate the antiproliferative effect of the ethanolic and aqueous extracts of
C. esculentus on MDA-MB-231 and MCF7 breast cancer cell lines. Also, the effects
of the extracts on cell cycle progression and cell migration activity of both cell lines
were studied. The possible mechanisms involved in the observed activity of the extract
were also elucidated. MTT assay was run to check the extract effect on the viability of
both cell lines, whereas flow cytometry was used to assess the cell cycle progression.
Wound healing and Matrigel assays were run to check the cell migration activity,
whereas qPCR and western blotting were run for gene and protein expressions,
respectively. The results show that C. esculentus extracts only reduced the cell viability
to 60 % at 24 hours and 80 % at 48 hours of treatment in MDA-MB-231 and MCF7,
respectively. Prolong treatment and increase concentration did not affect the cell
viability further.
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