Foroozandehasl, Seyedeh Parisa
(2019)
The Effect Of Hard Protein Corona On
Qd Nanoparticle Towards Senescent
Cells.
PhD thesis, Universiti Sains Malaysia.
Abstract
Nanomedicine is an important area of study that examines the utilization of
nanomaterials and nanoparticles in medical therapy and diagnostic purposes. However,
not many studies have focused on the aging related aspect of nanomedical research that
could have been valuable in treating aging associated diseases such as Werner syndrome,
sarcopenia and Alzheimer’s. In the present work, the cytotoxic potential of PEGylated
quantum dots (QD-PEG) and hard protein corona coated QD-PEG (QD-HC) on cells of
opposing age groups were examined. In the initial phase of the study, the interaction of
QDs with proteins from human blood plasma were analyzed. The results have shown that
protein corona was able to form on pristine QD-PEG based on SDS-PAGE, MALDITOF/
TOF, LC-MS/MS and μBCA analysis. Formation of hard protein corona had
transformed its physicochemical properties, which had in turn affected the colloidal
stability of QD-PEG in a significant manner. At proportionate levels of nanoparticle
concentration, hard protein corona had imbued distinct photonic and colloidal
characteristics to QD-PEG that were better suited for nanomedical applications in terms
of: (1) enhanced photostability at extreme pH conditions, (2) greater resistance to changes
in extracellular medium that induces agglomeration and gravitational sedimentation, and
(3) increased robustness to degradation and leaching of QDs’ core materials at extreme
pH conditions.
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