Yasin, Zahidah Nasuha Mohd
(2020)
Effect of accelerated portland cement-chitosan on dentinogenic/osteogenic differentiation of stem cell from human exfoliated deciduous teeth.
Masters thesis, Universiti Sains Malaysia.
Abstract
Mineral trioxide aggregate (MTA) is a recent gold standard endodontic biomaterial
but its application is limited by expensive cost, long setting time and can cause
cell toxicity. To overcome this problem, the discovery and development of new
potential biomaterial is crucially required. Hence, in this study we determined the
effects of
accelerated Portland cement-chitosan (APC-CHT) on the dentinogenic and
osteogenic differentiation in stem cells from human exfoliated deciduous teeth
(SHED). The APC-CHT was prepared 1 week prior to the treatment on SHED for 14
days. The cell
morphology was accessed using an inverted microscope. SHED demonstrated
phenotype transition from fibroblast-like to extended-spindle morphologies starting
from Day 7 until Day 14. The highest protein concentration obtained through
Bradford assay was 16.89 mg/ml which is in group 4 (APC-2.5 % CHT). The sizes
of proteins
in this study i.e. OCN, GAPDH, ALP, DMP-1, MEPE, OPN and DSPP were evaluated
using SDS-Polyacrylamide Gel Electrophoresis (SDS-PAGE). The highest
band intensity was observed in Day 14 in group 3 (APC-1.25 % CHT) and 4
(APC-2.5 %
CHT). Western blot analysis also demonstrated the appearance of apparent bands in
Day 14 in both group 3 and 4. In conclusion, APC-CHT exhibited favourable effects
on dentinogenic/osteogenic differentiation in SHED and has the potential to be used
as an alternative material in dental applications.
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