Zamberi, Nurul Nadia Mohamad
(2020)
Metastatic inhibitory effect of gallic acid combined with cisplatin on hela cells proliferation.
Other thesis, Universiti Sains Malaysia.
Abstract
Due to the multiple adverse effects of chemotherapy treatment, more researches with natural
product discovery has been conducted to treat various types of cancer. A polyphenol
derivative, gallic acid, was previously reported to combat the growth of cancer cells. Hence,
this study aims to assess the metastatic inhibitory effect of gallic acid combined with cisplatin
against HeLa cells. Cell migration, cell invasion and cell proliferation were measured by
scratch-wound healing assay, Transwell invasion assay and colony formation assay,
respectively. After 24-hour of scratch-wound healing assay, the combined treatment showed
significant decrease in wound closure rate where 43.08 ± 6.21% of the wound was filled by
the HeLa cells in comparison to control wound width. Combination of gallic acid and
cisplatin also exerted a significant decrease in the number of invaded cells at 44.55 ± 6.04%
as compared to the control group by Transwell invasion assay. Gallic acid and cisplatin were
able to significantly reduce the proliferation HeLa cells by colony formation assay. However,
the effect that was produced by their combination was greater than that of each agent alone.
Hence further study either in vitro and in vivo is required to strengthen the current findings
such as research regarding the involvement of PI3K/AKT/mTOR signaling pathway in
decreasing migration and invasion capability of HeLa cells.
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