Design And Evaluation Of Artificial Catalysts That Possess Enzyme-Like Amidolytic Catalysis

Wong, Yoke Ming (2017) Design And Evaluation Of Artificial Catalysts That Possess Enzyme-Like Amidolytic Catalysis. PhD thesis, Universiti Sains Malaysia.

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Abstract

In the present study, with the main goal of mimicking the amidase activity of protease,artificial polymer catalyst and peptide catalyst were designed and synthesized followed by various evaluations to investigate the validity of the design to possess enzyme-like amidolytic catalysis. Poly(N-isopropylacrylamide) microgel(NMG) has been developed by adding various functional groups to control surface charges, hydrophobicity, pKaand protein adsorption capacitybut seldom for enzyme mimics. Here, NMGsanchored with three types of functional groups were developed and studied aspolymercatalyststo hydrolyze amide bonds under evaluated mild conditions. Various evaluationstrategies were testedfor efficient hydrolysis activity on a p-nitroaniline-based substrate by using a colorimetric assay.

Item Type: Thesis (PhD)
Subjects: Q Science > QH Natural history > QH1 Natural history (General - Including nature conservation, geographical distribution)
Divisions: Pusat Pengajian Sains Kajihayat (School of Biological Sciences) > Thesis
Depositing User: Mr Aizat Asmawi Abdul Rahim
Date Deposited: 23 Nov 2020 02:00
Last Modified: 23 Nov 2020 02:00
URI: http://eprints.usm.my/id/eprint/47925

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