Mansor, Mohamad Hafizi
(2005)
The study of Interacting Partners of a
Transcription Factor, YB-1.
Masters thesis, Universiti Sains Malaysia.
Abstract
The transcription factor, YB-1 was considered to be a transcription factor. YB-1
also involved in many genes regulation, such as genes involved in cell proliferation
and cell growth, DNA repair, multi drug resistance, modification of chromatin, redoxstate
dependent-transcriptional enhancing, tissue cell specific processes, stress
response, immune response (major histocompatibility class II genes), and many more.
Because of several important of YB-1, my supervisor proposed to study of YB-
1 's interacting partners. Base on interacting partner of YB-1, we hopefully can explore
properties of YB-1.
In this study, we used culture ofHeLa cells, and lysis the cells using Single-step
lysis for extraction of total cellular protein, and nuclear and cytoplasmic
proteinextraction methods to get YB-1 protein. Using co-immunoprecipitation analysis
and several antibodies, we will detect interacting partners of YB-1.
Finally after co-immunoprecipitation analysis, we got YB-1 protein has
specific interaction with P-glycoprotein mdr 1 protein which involved in multidrugresistant
cancer cells.
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