Fauzi, Haslinda Mohd
(2005)
Evaluative study of platelet apheresis by
haemonetics MCS+ and comparison with random
donor platelets.
Project Report.
Pusat Pengajian Sains Kesihatan, Universiti Sains Malaysia.
(Submitted)
Abstract
Platelet concentrates (PCs) can be prepared by two methods, including apheresis
and random donor platelets (RDP). To ensure good quality of PCs, standards have been
set up by Transfusion Medicine Unit and according to these apheresis PCs should have
platelet count more than 2. 0 x 10 I I I Unit, volume more than 40mL per 60 x I 09 platelets
and white blood cell (WBC) contamination less than 0.5 x 1091 Unit. Similarly RDP
should have platelet count more than 55 x 1091 Unit; volume within 40 to 60 mL, WBC
contamination should be less than 0.5 x 109 I Unit and red blood cell (RBC)
contamination should be less than 1.0 x 1091 Unit. For both platelet apheresis and RDP
there should be no growth of microorganism. The main objective of this study was to
determine whether the standards were met or not and to compare the different parameters
including contamination with bacteria, WBC and RBC between RDP and apheresis. To
meet our objectives we designed a prospective study to be conducted in Transfusion
Medicine Unit and 47 apheresis PCs and 47 RDP samples were collected. We also
studied the effects of apheresis on pre- and post-donation full blood count (FBC),
hemolysis and side effects experienced by 50 donors using Haemonetics MCS+. Results
of our study showed that the criteria for apheresis PCs and RDP were met except for
RBC contamination in both techniques and volume in RDP, WBC and RBC
contaminations in apheresis PCs were unexpectedly higher than in RDP, while bacterial
contamination was lower in apheresis PCs. Comparison of pre- and post donation counts
showed, significant decrease in platelet count while WBC, hemoglobin and hematocrit
levels were significantly increased. In general no hemolysis was observed in apheresis
donors by Haemonetics MCS+. Citrate toxicity was mild but it was the most common
side effect during apheresis donation. We concluded that quality of apheresis PCs and
RDP met the standards except for RBC contamination, volume of RDP and bacterial
contamination. Platelet apheresis by Haemonetics MCS+ is a safe, reliable and effective
method.
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