Halim, Farah Amani Abdul
(2018)
Pretreatment Of Oil Palm Frond By
Phanerochaete Crysosporium Ck01 And
Enzymatic Hydrolysis For Ethanol
Production By Saccharomyces Cerevisiae Hc10.
Masters thesis, Universiti Sains Malaysia.
Abstract
Oil palm frond (OPF) is one of the most abundant waste generated from an oil
palm plantation. OPF can be utilized as a feedstock for bioethanol and can
simultaneously address its disposal issue in the plantation. In this project, OPF was
divided into two types of feedstock; biomass and juice by mechanical pressing. Firstly,
the dried biomass was biologically treated using white-rot fungi; Phanerochaete
chrysosporium CK01 for biodelignification. Two parameters were tested; effect of
inoculum size and fermentation duration. The best parameters were inoculum size of
1.0x106 spore/mL and 3 weeks of fermentation duration which gave 27.87% of
delignification. In the second stage, the treated biomass was subjected to enzymatic
hydrolysis by either individual or in combination to produce a sugar hydrolysate. Two
parameters tested were enzyme loading (in individual enzymatic hydrolysis) or
enzyme ratio (in combined enzymatic hydrolysis; Cellulase A “Amano” 3 and
Hemicellulase “Amano” 90) and hydrolysis time. The result shows that the combined
(cellulase and hemicellulase) enzymatic hydrolysis produces higher amount of simple
sugar (5.15g/L) using a combination of enzyme ratio of 1:4 (cellulase:hemicellulase)
with a hydrolysis time of 120 minutes compared to individual enzymatic hydrolysis
(3.26g/L). Prior to beginning of the third stage, the amount of simple sugar in the
hydrolysate (OPFH) and juice (OPFJ) were compared using high performance liquid
chromatography (HPLC) analysis.
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