Yusoff, Nik Yusliyana Nik
(2018)
An in vitro study on the antimicrobial property of Malaysian honey and propolis extracts against microorganism implicated in denture stomatitis.
Masters thesis, Universiti Sains Malaysia.
Abstract
Denture stomatitis presents as a chronic disease commonly occurs under
maxillary prosthesis. Despite the existence of a great number of antibacterial and
antifungal agents, treatment failure is observed frequently. An alternative solution is to
find material that has antibacterial and antifungal properties of equivalent nature to the
action of miconazole and chlorhexidine. It must be well tolerated by the body, causing
no side effects or interference with drug metabolism. Honey and propolis seem to fit all
the criteria mentioned above. Honey and propolis have anti-inflammatory, antimicrobial,
antioxidant and anticancer properties. The aim of the current study was to investigate the
physical andantimicrobial properties of honey and propolis extracts of Apis dorsata,
Apis mellifera and Trigona spp. against common microorganisms implicated in denture
stomatitis. The physical properties of honey were examined based on colour, acidity and
moisture content. The efficacy of honey and propolis extracts towards microorganisms
was evaluated by using agar well diffusion assay. The effective concentrations of the
honey and propolis extracts were determined by Minimum Inhibitory Concentration
(MIC). In physical test, the data are presented as mean and standard deviation of each of
the attributes. For agar well diffusion assay, three-way ANOVA followed by post-hoc
comparison was used for statistical analysis (p < 0.05). For determination of minimum
inhibitory concentration, the reading from ELISA reader and visual observation werereported on the concentration of honey and propolis extracts. The results showed that
Malaysian honey exhibited better acidity and highest moisture content than the
benchmark, Apis mellifera honey. For result of agar well diffusion assay, Trigona spp.
honey exhibited favourable antimicrobial activity against several microorganisms with
large inhibition zones against Actinomyces sp and Candida tropicalis at 25.79±0.32 mm
and 12.29±0.23 mm, respectively. For MIC value of honey, Trigona spp showed lowest
MIC value against Staphylococcus aureus and Actinomyces spp. at 62.5mg/ml while
against all Candida spp. at 250mg/ml. The propolis extract from Trigona spp.exhibited
better antibacterial activity compared to propolis extract from Apis mellifera against S.
aureus and Streptococcus mitis at 13.58±0.90 mm and 14.05±0.13 mm, respectively
with no antifungal activity against Candida spp. For MIC value of propolis extracts,
Trigona spp. showed lowest MIC against S. aureus and S. mitis at 62.5mg/ml while
500mg/ml against all Candida spp.The MBC value was higher than MIC value for
honey and propolis. Hence, it can be concluded that Malaysian honey and propolis
extract mainly Trigona spp. exhibited better antimicrobial activity than Apis mellifera
against microorganisms implicated in denture stomatitisand have potential usage in the
treatment of common oral diseases.
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