Tumin, Norizah
(2004)
Antibacterial activity of some selected
brands of Malaysian honey.
Other thesis, Universiti Sains Malaysia.
Abstract
Honey is an ancient remedy for the treatment of infected wounds, which has
recently been 'rediscovered' by the medical profession, particularly where
conventional modern therapeutic agents are failing. Honey is made up of simple
sugars and is an excellent source of energy. It is hypertonic and sterile with
antibacterial activity against many pathogenic bacteria.
The present study aims to investigate the antibacterial activities of five different
brands of Malaysian honey. 'Tualang', 'Hutan' and 'Gelang' honey are produced
from wild bee, 'Pucuk Daun' and 'Ee Feng Gu' honey are from honeybee farm
were considered for this study. Six different pathogenic bacteria, E. coli, S. typhi,
S. sonnei, P. aeruginosa, S. aureus and S. pyogenes were selected as test
organisms. Diluted and neat honeys were used for the antibacterial assay.
Solvent extraction of honey was carried out with an aim to isolate the active
principle behind the antibacterial property. MIC of the selected honey was
determined.
'Tualang' honey was found to be most effective against S. typhi. It also showed a
substantial antibacterial activity against E. coli and S. pyogenes. 'Hutan' and
'Gelang' honey does not show any antibacterial activity against the organism
tested. 'Pucuk Daun' honey showed antibacterial activity E. coli and S. sonnei.
'Ee Feng Gu' was active against E. coli, S. typhi, S. sonnei and S. aureus.
Among the solvent extracts only acetone fraction showed antibacterial activity.
Extracts with petroleum ether, chloroform and ethyl acetate does not show any
activity.
The MIC (Minimum Inhibitory Concentration) values of 'Pucuk Daun', 'Gelang' and
'Hutan' honey were 5.0 IJQ/ml, 17.5 IJQ/ml and 28.75 IJQ/ml respectively against S.
sonnei.
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