Hashim, Nur Hamizah Abdul Ghani @
(2011)
Modeling And Optimization Of Isoamyl Acetate Production In Enzyme Catalyzed Esterification.
Masters thesis, Universiti Sains Malaysia.
Abstract
Isoamyl acetate is an important substance which is used in various industries
including flavour and fragrance industry. The production of this compound using
enzymes had attracted many attentions due to its advantages of moderate operating
parameters, limited side reactions and easy product recovery. In enzyme catalyzed
esterification, acetic acid and isoamyl alcohol are used as substrates to the Candida
antarctica immobilized lipase to produce isoamyl acetate. In direct esterification
reaction, the ester synthesis is affected by excess water which can change the
thermodynamic balance of reaction towards hydrolysis. On the other hand, during the
initial stage of the reaction, water activates the enzymes and increases enzyme
activity thus increases the reaction rates. Therefore, the amount of water content has
been a critical parameter in enzyme catalyzed esterification. Beside this, few
parameters considered to affect the process were temperature, enzyme loading,
concentration of substrates and reaction time. In order to understand the process and
the relationship of these selected parameters, process optimization and model
development are essential. The processes optimizations were done via “one-factor-ata-
time” and response surface methodology (RSM). The optimum conditions obtained
from “one-factor-at-a-time” were compared with RSM and the results were in range.
The coefficient of determination, R2 of the RSM model was 0.9448.
Actions (login required)
 |
View Item |
