Lim, Phaik Siew
(2011)
mRNA Expression Of Bone
Morphogenetic Protein 7 (bmp7) During
Folliculogenesis In Zebrafish.
Masters thesis, Universiti Sains Malaysia.
Abstract
The potential roles of bone morphogenetic protein 7 (bmp7) in ovary have drawn
increasing attention. Present study aims to investigate the expression pattern of bmp7a and
bmp7b in zebrafish. Adult female tissues were sacrificed for sampling of brain, eye, gill,
heart, intestine, kidney, ovary, liver and swim bladder. For follicle compartments, follicle
layers were mechanically separated from oocytes after cold-shock treatment. To verify
analysis of bmp7a and bmp7b expression during different follicle stages, previtellogenic;
early- and mid-vitellogenic mixed; full-grown immature at daytime, 0300 h, 0400 h;
maturation (0500 h) and ovulation (0600 h) stage were isolated from dissected ovaries.
Using semi-quantitative real-time PCR, bmp7a was found to be abundantly expressed in
ovary whereas bmp7b was highly expressed in swim bladder, brain, eye, gill and ovary. Both
molecules were predominantly expressed in the follicle layers rather than the oocyte. During
folliculogenesis, bmp7a and bmp7b were expressed highly at previtellogenic stage.
Thereafter, bmp7a remained rather constant across follicle developmental stages. In contrast,
bmp7b expression progressively reduced to extremely low level at ovulation across the
developing follicles. Overall, this finding indicated the possible importance of bmp7b as an
autocrine/paracrine growth factor for follicular development at previtellogenic and, as
repressor of oocyte maturation during folliculogenesis.
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