Shaheran, Anish Ameera
(2018)
Determination of toxicity and antioxidants on white edible bird nest (Aerodramus fuciphagus) from different district in Kelantan.
Masters thesis, Universiti Sains Malaysia.
Abstract
Edible bird nest (EBN) is one of the natural products that have high therapeutic
potential and pharmacological properties. Therefore, this study aimed to determine the
toxicity and antioxidants activity on white edible bird nest (Aerodramus fuciphagus)
from different district in Kelantan (Tanah Merah, Tumpat and Jeli). The samples were
extracted using three solvents which are distilled water, sodium hydroxide and
hydrochloric acid. The toxicity of the EBN samples with different solvent of extraction
were tested using MTT assay against cancerous (HeLa and MCF-7) and non-cancerous
cell line (SVG p12). Minerals content in EBN was studied using inductively couple
plasma mass spectrometry (ICP-MS) and the other toxicity test was brine shrimp
lethality assay (BSLA) for LC50 values. The antioxidants activity was tested on EBN
samples extracts using DPPH radical scavenging assay and Ferric reducing power
assay (FRAP). EBN samples extracts showed higher inhibition activity against MCF-
7 cell line. Meanwhile, three samples of EBN extracts induced cell proliferation on
SVG cell line as the IC50 value more than 100%. Minerals content in all EBN samples
were below the maximum regulatory limit as suggested by FDA, WHO and SIRIM
guidelines except sodium content that slightly higher than 2 ppm. The results showed
no LC50 value was determine on EBN against brine shrimp. The antioxidants activity
using DPPH and FRAP assay showed that NaOH extracts from three sample EBN had
the lowest IC50 values and the highest reducing power. Thus, the results finding
showed that the EBN samples extracts can be considered as cancer-preventive agents
and are relatively safe to be consumed. Extracts of EBN samples can be considered asantioxidants agent. However, further validation have to be performed to understand
the active compounds that poses safety, toxicity and antioxidants activity of EBN.
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