Wahab, Dayang Yasmin Abang Abdul
(2018)
Elucidating the toll-like receptor 4 involvement in striatum and cerebellum of swiss albino adult mice on motor and sickness behaviours.
Masters thesis, Universiti Sains Malaysia.
Abstract
Alcohol addiction is one of the possible factors in stimulating brain microglia
activation and leading to neuroinflammation through toll-like receptors (TLR) which
are present in microglia. In fact, alcohol addiction ultimately causes motor deficits
through neuroinflammation. However, the underlying mechanisms of
neuroinflammation inducing motor behaviour through activation of TLR4 receptors
have not yet been elucidated. TLR are always found to be associated or involved in
the induction of neuroinflammation in neurodegenerative diseases. TLR4 is stimulated
by TLR4 Agonist, Lipopolysaccharide (LPS), and the TLR4-LPS interaction has been
found to result in physiological and behavioural changes including retardation of
motor activity in the mouse model. Therefore, the present study aimed to investigate
the locomotor behaviour, gene expression of serotonin receptors (HTR1A and
HTR2A), dopamine receptors (Dopamine D1 receptor and Dopamine D2 receptor) and
glutamate transporters (EAAT1 and EAAT4) in the striatum and cerebellum following
treatment with TLR4 agonist. The animals were divided into three groups; (1) Control
(n=12), (2) LPS treatment (0.83mg/kg) (n=12) 6 h and (3) LPS treatment (0.83mg/kg)
(n=12) 24 h. After treatment, locomotor behaviour was analysed in open field test,
wooden beam test and hanging test at 6 and 24 h post-LPS administration. Following
behaviour test, animal’s brains were harvested and striatum and cerebellum isolated
for gene expression studies. Results showed that there were locomotor deficits at 6 h
but not in 24 h. The gene expression studies suggested that there were significant
changes in serotonin receptors (HTR1A and HTR2A), dopamine receptors (Dopamine
D1 receptor and Dopamine D2 receptor) and glutamate (EAAT1 and EAAT4)
transporters in the striatum and cerebellum along with motor deficits. In conclusion,TLR4 possibly causes motor deficits through regulation of glutamate transporter
EAAT1 in striatum and cerebellum.
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