Rasool, Aida Hanum Ghulam and Rahman, Abdul Rashid Abdul
(2004)
Method optimisation and validation study on use of laser doppler flowmetery to assess microvascular endothelial function.
Method optimisation and validation study on use of laser doppler flowmetery to assess microvascular endothelial function.
(Submitted)
Abstract
Introduction: Human postocclusive forearm skin reactive hyperemia is not only a potential means of identifying early signs of
cardiovascular diseases, it can also be used in the assessment of local microvascular response to topically applied compounds on skin. The
method is not fully characterized. In this study, we investigated the influence of occlusion time on postocclusive forearm skin reactive
hyperemia using laser Doppler fluximetly (LDF). Methods: Twenty healthy male volunteers were studied on three separate days (at least 24
lti h apart) via a randomized design. Volunteers were studied in a supine position while fasted. Laser Doppler probes were placed on the volar
surface of the antebrachium. In preliminary studies, 3 min of upper arm blood flow occlusion at suprasystolic pressure was found to be the
upper limit of tolerability. Subsequently, volunteers were randomized to receive 1, 2, or 3 min occlusion on 3 different days. Skin blood flux
1~ was measured before, during, and after occlusion using LDF. The primary outcome calculated was maximal change in ski11 blood flux before
and after occlusion, expressed in arbitrary units (AU). Results: Skin blood flux changes (mean ± S.E.M.) after 1, 2, and 3 min occlusion
period were 15.39 ± 1.27 AU, 24.84 ± 1.62 AU, and 32.14 ± 1.73 AU, respectively. Using repeated-measures analysis of variance
(ANOVA), significant difference (P<.OS) in skin blood flux changes were revealed between these three occlusion durations, where 3 min
occlusion produced significantly greater in skin blood flux occlusion change compared to I and 2 min occlusion. Conclusion: Three minutes
of occlusion produces the greater postocclusive reactive hyperemia. It is recommended that studies using postocclusive forearm skin reactive
hyperemia should occlude the forearm for at least 3 min.
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