Noordin, Liza and San, Gregory Tan Jin and Othman, Mohd. Shukri
(2004)
Effect of peritoneal fluid on early embryonic
development.
Effect of peritoneal fluid on early embryonic development.
(Submitted)
Abstract
The aetiology of endometriosis associated with infertility remains poorly
understood. In recent years, the potential influence of peritoneal fluid (PF) or its
cellular components have been proposed as possible mediators of infertility in
endometriosis through it toxic effects on pre-implantation embryo. Several
factors have been identified as embryotoxic factors, however, the mechanism
of embryotoxicity have not well clarified. Studies were therefore undertaken to
clarify the possible mechanisms of embryotoxicity in endometriosis and to
determine the possible embryotoxic factor(s) that involved in the process. The
role of exogenous nutrient (pyruvate) has also been examined in reducing or
eliminating the embryotoxicity. Peritoneal fluid was collected from infertile
women at reproductive age with 21 endometriosis (PF-E) (7 minimal or mild, 7
moderate, 7 severe) and 7 without endometriosis (PF-NE). Addition of PF-E to
the culture medium from all stages of endometriosis significantly suppressed
the mouse embryo growth at all stages of development, at 24, 48 and 72 hours,
which correlated with the severity of the disease, as compared to control (no
peritoneal fluid). Excessive pyruvate was able to reduce the embryotoxicity at
all stages of development in minimal or mild and moderate PF-E, and only
during the first 24 hours in severe PF-E. The level of IL-6 concentration was
significantly higher in PF-E as compared to PF-NE and correlated with the
severity of the disease. However, no significant difference was noted in the
level of IL-8 between the two groups. Both interleukins were found to be
embryotoxic with IL-6 is more potent. A positive correlation between the levels
of IL-6 and its embryotoxicity was noted. These findings propose embryotoxicity
to be a possible mechanism of infertility in endometriosis and the growth
promoting effects of pyruvate indicated the mechanism of embryotoxicity might
involve functional disruption of the intermediary metabolism such that
excessive pyruvate is required to maintain embryo growth.
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