Iqbal, Muhammad Shahid
(2013)
Investigation Of Drug Nutrient Interactions And
Physicochemical Compatibilities Of Ceftazidime,
Cefotaxime And Cefepime In Neonatal Total
Nutrient Admixtures Using Hplc.
PhD thesis, Universiti Sains Malaysia.
Abstract
and trace elements). The stability of the formulations were evaluated for various physicochemical
parameters such as pH, globule size, zeta potential, precipitation, flocculation,
coalescence and concentrations of sodium, potassium and drug concentrations. The experiments
were carried out at room temperature (25±2°C) and samples were collected at 0, 4, 8, 16 and 24
hours respectively. Antibiotic concentrations in the samples were analyzed by using the newly
developed and validated HPLC methods. SPE was used by modification of the mobile phase and
washing solvents in different ratios to achieve better accuracy, precision, linearity, earlier
retention times, clear elution and recovery. The study showed that ceftazidime and cefepime
were stable for up to 16 hours while cefotaxime was stable for up to 24 hours in all TNAs
formulations and there after degraded more than 10% respectively. Physico-chemical
compatibility results showed that there were no significant changes in pH, globules size, zeta
potential and concentrations of sodium and potassium in all of the formulations (all p >0.05). In
addition, there was no precipitation or flocculation observed in any of the formulation. The
newly developed HPLC methods were able to detect selected cephalosporins more accurately,
precisely, effectively and efficiently. In conclusion, this study showed that ceftazidime and
cefepime were compatible and stable for 16 hours in/with TNAs while cefotaxime was
compatible and stable for 24 hours in/with TNAs.
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