Letchumanan, Mobana
(2004)
Expression of the transcription factor, PP ARs in immune cells of balb/c mice.
Other thesis, Universiti Sains Malaysia.
Abstract
Peroxisome proliferator activated receptors (PP ARs) are ligand-activated transcription
factors that belong to the nuclear hormone receptor superfamily. Despite significant
involvement in the lipid metabolism, little is known about the expression pattern and the
role of different PP ARs in immune regulation. Emerging evidence suggests that PP ARs
may act as negative immunomodulators. To provide further insight into the role ofPPARs
in the immune system, the expression patterns of PPARa., PPARyl and PPARy2 in
peritoneal macrophages and splenocyte-derived T lymphocyte subsets, CD4+ and CD8+
cells of balb/c mice were studied.
Total RNA from each of the cell subsets was extracted, reverse-transcribed to eDNA and
the expression of PPARa, PPARyl and PPARy2 quantified by Real-Time PCR using
specific primers and probes. The expression levels were then calculated per J..lg total RNA
and statistically analyzed using statistical software. In addition, cytokine expression
profiles in the selected immune cells were also assessed using multiplex PCR method.
The results obtained in the present study demonstrate that all the immune cell subtypes
predominantly express PP ARyl suggesting the probable involvement of this transcription
factor in immune response or other biological functions of these cells. The study also
further confirms that lymphocytes express PP ARa. CD8+ lymphocytes showed the lowest
expression of all PP AR isoforms compared to other immune cells suggesting minimal
involvement of the PP ARs in the immune response mediated by these cells. In addition
baseline expression levels of various cytokines in immune cell subsets in young adult mice
also were showed in the current study.
Actions (login required)
 |
View Item |
