Ahmad, Nor Hazwani
(2013)
Effects Of Catharanthus Roseus Aqueous
Extract On Jurkat Cells And Normal
Peripheral Blood Mononuclear Cells.
PhD thesis, Universiti Sains Malaysia.
Abstract
The objective of the present study was to evaluate the effects of crude aqueous extract of
C.roseus leaves on Jurkat cell line (leukemic T-cells) and normal peripheral blood
mononuclear cells (PBMCs).
Analytical high performance liquid chromatography (HPLC) was used to obtain the
concentration of vinblastine in the C.roseus extract while the identification of selective
compounds was determined by comparing the UV spectra of each compound with
standards of compounds previously identified in C.roseus. The analytical data indicated
the concentration of vinblastine was 1636 ± 0.2 μg g-1 of aqueous extract, equivalent to
445.6 ± 0.06 μg g-1 of dried leaves. Additionally, four of the alkaloids were identified as
serpentine, vindoline, catharanthine and vindoline.
The cytotoxicity effects of the extract on Jurkat cells and normal PBMCs were first
examined by MTS [3-(4,5-dimethylthiazol-2-yl)-5(3- carboxymethoxyphenyl)-2-(4-
sulfophenyl)-2H-tetrazolium] assay. The C.roseus extract was found significantly
inhibited the proliferation of the Jurkat cells and also induced the proliferation of normal
PBMCs. The cytotoxicity effect on Jurkat cells was demonstrated with IC50 values of
2.55 μg/ml and 2.38 μg/ml at 48 h and 72 h, respectively. Maximal cell proliferation was
observed in PBMCs incubated with 1000 μg/ml of the C.roseus extract at various
incubation times for different blood donor.
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