Generation And Characterization Of Rna Aptamer Against rHuEPO-a By Selex Technology

Marimuthu, Citartan (2014) Generation And Characterization Of Rna Aptamer Against rHuEPO-a By Selex Technology. PhD thesis, Universiti Sains Malaysia.

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Abstract

Systematic Evolution of Ligands by Exponential Enrichment (SELEX) enables the isolation of aptamer (ssDNA or RNA that possesses high binding affinity and specificity against virtually any target molecules). In this study, a RNA aptamer against recombinant human EPO-alpha (rHuEPO-α) was successfully isolated by SELEX technology. After 11 cycles of SELEX, cloning and sequence analysis showed an appearance of a single major clone constituting the putative RNA aptamer, termed REPORA-6, with a dissociation constant of 25±1 nM.

Item Type:	Thesis (PhD)
Subjects:	R Medicine > R Medicine (General) > R735-854 Medical education. Medical schools. Research
Divisions:	Institut Perubatan & Pergigian Termaju (Advanced Medical & Dental Institute (AMDI)) > Thesis
Depositing User:	ASM Ab Shukor Mustapa
Date Deposited:	22 Jul 2019 03:03
Last Modified:	22 Jul 2019 03:03
URI:	http://eprints.usm.my/id/eprint/44994

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